Fplc akta prime software

The akta pure is a flexible and intuitive chromatography system. The akta software is complex, but once you figure out how to use all the submenus and screens it isnt a big deal, although it is more tedious to setup users and runs, initially. This high flow rate is necessary when working with. The ge akta prime fplc is a compact onestep purification liquid chromatography system that operates with most compact columns. Programs are accessible through a front panel lcd display. Whats the difference between the akta prime and akta prime plus. Ge akta prime plus liquid chromatography system gmi.

Aktaprime plus cytiva, formerly ge healthcare life sciences. See appendix a for overview scheme of the akta purifier customized system in zarivach laboratory. Amersham biosciences akta prime fplc system marshall scientific. Akta pure cytiva, formerly ge healthcare life sciences. Akta pure 25 purification system is intended for microgramscale protein purifications in tricorn columns up to milligramscale using hitrap and hiscreen columns. Ge akta explorer certified fplc system gmi trusted lab. Full programming control is available for almost any imaginable purification. Flow rates up to 50 mlmin max pressure 1 mpa akta prime consists of a pump, fraction collector, uv monitor, conductivity monitor. The akta prime is also designed with the akta prime view 5. Page 15 a complete control software package for supervision of aktaprime plus automated liquid chromatogra phy systems. Pontis, in methods for analysis of carbohydrate metabolism in photosynthetic organisms, 2017. Updating ges akta avant to windows 10 michaels domain. Primeview is a complete control software package for supervision of aktaprime automated liquid chromatography systems. Feb 10, 2012 10 things you need to know about biochromatographuy fplc vs hplc duration.

Ge akta prime liquid chromatography system gmi trusted. Unicorn 7 features an intuitive interface for creating methods by dragging and dropping predefined phases for a wide range of techniques. Protein purification the aim of this tutorial is to demonstrate the use of the ubiquitous akta systems and prepacked hitrap chromatography media for rapid, mediumscale protein purification. Equipnet is the worlds leading provider of used fplc equipment and various other equipment. This innovative and versatile ge fplc system is engineered for high quality gel filtration, hic, affinity chromatography, buffer exchange, and ion exchange. Used akta explorer, used akta pilot, usedused akta. Aktaprime plus is a chromatography system that performs simple. Ge healthcare ge healthcare akta fplc purifier system for sale as a parts unit. The akta prime system operates efficiently with most prepacked columns such as hitrap, hiprep, and hiload columns which easily snap on to the side of the unit. Fast protein liquid chromatography fplc is a type of liquid chromatography that provides high resolution by smalldiameter stationary phases for protein characterization and. The ge akta prime plus fplc system is flexible for more general. With the use of the fplc, one can separate and characterize protein mixture as well as pure proteins. With the use of the fplc, one can separate and characterize protein. The akta prime plus offers high reproducibility in a compact and easytouse design with preprogrammed templates.

Akta go is a small and compact liquid chromatography system that. Cytiva formerly ge healthcare life sciences 3,031 views 3. This innovative and versatile ge fplc system is engineered for high quality gel filtration, hic, affinity chromatography, buffer exchange, and ion exchange the akta prime is also designed with the akta prime view 5. Amersham biosciences akta prime fplc system marshall. Amersham biosciences akta prime fplc system the amersham biosciences akta prime is a compact, singlestep fplc system designed for simple purification of proteins. Items as shown upc900 p920 inv907 iv908 m925 pv908 power cord does not include software but we can add a computer wit. Air in the pumps can really mess up your runs, so you can manually prime the pumps with a syringe to pull through any trapped. Akta go protein purification system cytiva, formerly ge. For fast protein liquid chromatography of tens of grams of protein, akta pure 150 can be used with flow rates up to 150 mlmin. After initializing the akta and the unicorn software go to the system control window, then to the manual menu and press option 1 pump, this display the system pump instructions window from where. The biorad software is very simple and easy to use right out of the box but doesnt offer as many tools, which some people dont care about anyway. The avant is the flagship of ge healthcares akta line of fplc fast. I cleaned my column with naoh, water and then i equibrilated it with buffer.

Ge akta prime plus fplc liquid chromatograph available new in crate at the lab world group. The fast performance liquid chromatography fplc is essentially a computerizedcontrol pump with a high degree of column selection. Ge healthcare akta purifier 10 fplc system youtube. Axelerist can purchase any equipment you need for your labs, and lease it back to you for less than the total cost of the equipment letting you devote more resources to building your business while ensuring you have access to the latest lab equipment. I was working with ge superdex 200 column on akta prime plus system. How can i use akta protein purifier and unicorn software. Biologic duoflow medium pressure chromatography systems are offered with multiple system configurations, optional upgrades, and a common software platform.

Home services the leaders in akta prime service, support and maintenance the leaders in akta fplc service, support and maintenance designed for a simplified process of protein purification at the laboratory scale, the akta fplc offers a variety of advantages over traditional, manual systems. Software to support purification using akta automated. Akta prime fplc options off topic ccp4 bulletin board. Both prime products work with primeview software, not unicorn, as the larger akta systems do. Primeview runs on a pc under microsoft windows 2000 or microsoft windows xp. The ge akta prime plus fplc system is flexible for more general purification tasks, such as ion exchange, affinity chromatography, buffer exchange, hic, and gel filtration. Find great deals on a selection of akta explorer fplc systems on labx. We recently purchased it and are pretty dissatisfied so far, the software isnt working rightit wont even install on the computer we bought from a 3rd party and to be. It also contains an overview of the safety instructions. Axelerist can purchase any equipment you need for your labs, and lease it back to you for less than the total cost of the equipment letting you devote. Component documentation documentation for components produced both by ge healthcare and by a thirdparty are, if existent, also included in the document package. After serving the market for many years, aktaprime plus was discontinued on 30 june 2019.

Items as shown upc900 p920 inv907 iv908 m925 pv908 power cord does not include software. Akta prime fplc fplc sytem ge, amersham injection, gradient, and buffer selection valves. The ge akta prime plus is a compact and versatile onestep purification system designed to perform general purification tasks including affinity chromatography, buffer exchange, ion. Samples can be injected in a range of 1 to 150 ml using syringes of 10ml, 50ml and 150ml. This fplc system offers an easytouse interface with preprogrammed templates, contributing to its high reproducibility and accuracy. Weve tried powering off the akta system, as well as shutting down the pc, but our cu950 is unable to connect to the akta system for us to connect using the unicorn software. Software to acquire and evaluate data from akta akta prime plus column chromatography equipment used. Gloves and protective eyewear should be worn when handling samples and reagents buffers, however it is preferable that the user. When the user selects a column, the akta explorer instinctively sets the column. Ge akta pure operating instructions manual pdf download.

Biologic duoflow mediumpressure chromatography systems. In these, higher pressures damage the chromatography medium. Nov 29, 2017 weve tried powering off the akta system, as well as shutting down the pc, but our cu950 is unable to connect to the akta system for us to connect using the unicorn software. Amersham biosciences akta prime fraction collector fplc chromatography. Suitable for analytical and preparative chromatography. Getting started with unicorn 5 boyce thompson institute.

Fplc uv detector cambridge scientific used lab equipment. I want to fix a superose 6 10300 gl column to our akta prime system. The ge aktaprime plus fplc system pump produces flow rates of up to 50 ml per minute with pressures up to 1mpa. Unicorn 7 cytiva, formerly ge healthcare life sciences. Using the akta prime plus october 22, 2012 3 j e n n i f e r a r r u d a attaching the column and washing the ethanol from the column. Protein purification laboratory of molecular biology. Our exclusive contracts with our clients yield a wide range of used fplc equipment from a number of respected oems, including ge healthcare, pharmacia biotech, amersham pharmacia biotech inc, and many others. However, the product info says that the superose 6 10300 gl is not suitable for use with the akta prime plus system. Akta explorer systems include an extensive column library with details of run parameters for each prepacked column. The ge akta prime plus is a small all in one protein purification system which runs general tasks including buffer exchange, affinity chromatography, gel filtration, hic and ion exchange. Aktaprime plus chromatography systems were launched in year 2001 and have been used by researchers in thousands of laboratories around the world. Im having difficulty finding any other fplcs recommended for research lab scale protein purification.

The akta prime plus fplc comes preprogrammed with common use methods like sample cleanup, tagged protein affinity purification and mabs, in simple to use formats. This unit is used for fast purification of proteins, peptides, and nucleic acids from microgram levels to tens of grams of target product. Page 1 page 2 table of contents page 3 page 4 introduction page 5 about this manual page 6 important user information page 7 page 8 regulatory information page 9 eu directives page 10. Column commonly, a chromatographic column is a tube made by glass which has varied diameter, from 5 mm to 50 mm and height from 5 cm to 1 meter. This makes loading and unloading chromatography column a cinch.

Once the lines have been washed with water, you will need to start a manual run before attaching your column to the system. Amersham biosciences ge fplc system cambridge scientific. Page 1 page 2 table of contents page 3 page 4 introduction page 5 about this manual page 6 important user information page 7 page 8 regulatory information page 9 eu directives page 10 eurasian customs union page 11 regulations for usa and canada page 12 other regulations and standards page page 14 associated documentation. Home products chromatography fplc system ge akta prime plus advanced search keywords. Basically, akta go and unicorn software will provide you with. For my research, i must use akta protein purifier and unicorn software but i dont understand them. Before starting an akta run, check all the tubing for bubbles.

Fplc systems lab equipment leasing is a smart move. Fast protein liquid chromatography fplc systems with autosamplerfraction collectors. Unicorn is a complete package for control and supervision of chromatography systems. Jan 25, 2017 akta go protein purification system and unicorn software. Akta pure, protein purification your way april scharfstein. Equipped with a front panel lcd display and easytouse push buttons, the ge akta prime plus offers high reproducibility and user convenience. Akta fplc mixer 925 problem and solution showing of 3 messages. Used akta explorer, used akta pilot, usedused akta purifier.

Amersham pharmacia biotech akta prime flpc system guaranteed. Chromatography software cytiva, formerly ge healthcare. With flow rates of up to 25 mlmin and pressure of up to 5 mpa, you are all set for affinity, size exclusion, or ion exchange chromatography. The fplc system works with hitrap, hiload and hiprep columns which easily snap into the side of the instrument. Ge akta prime plus fplc system available at the lab world group. Ge aktaprime plus operating instructions manual pdf download. Ge healthcare akta fplc purifier system for sale as a parts unit. Akta go has been developed for automated chromatography from the heritage of our fast protein liquid chromatography fplc technology. Ge akta prime plus fplc system marshall scientific.